A validated gene regulatory network and GWAS identifies early regulators of T-cell associated diseases (exon array)

ABSTRACT: In this work we present an analytical strategy to systematically identify early regulators by combining gene regulatory networks (GRN) with GWAS. We hypothesized that early regulators in T-cell associated diseases could be found by defining upstream transcription factors (TFs) in T-cell differentiation. Time series expression and DNA methylation profiling of T-cell differentiation identified several upstream TFs, of which TFs involved in Th1/2 differentiation were most enriched for disease associated SNPs identified by GWAS. Peripheral blood mononuclear cells (PBMCs) were prepared from fresh blood from 10 patients with seasonal allergic rhinitis and 10 healthy controls using Lymphoprep (Axis-Shield PoC, Oslo, Norway) according to the manufacturer’s protocol. PBMCs were stimulated with a

ORGANISM(S): Homo sapiens

SUBMITTER: Danuta Gawel 

PROVIDER: E-GEOD-60677 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress