Dicer WT/KO MSC RNA-Seq [polyA RNA]

ABSTRACT: RNA-Seq performed on Dicer KO and WT murine mesenchymal stem cells MicroRNAs (miRNAs) are small non-coding RNAs that regulates development and disease but induce only moderate repression of directs mRNA targets, suggesting that they coordinate with other modes ofs cellular regulation to effect large changes in gene expression. Ins this work we decouple direct effects of global miRNA loss froms transcriptional changes downstream in a pair of isogenic murines fibroblast cell lines with and without Dicer expression. Wes demonstrate how effects on direct miRNA targets are amplified bys transcription machinery through the construction of a network models that identifies specific transcription factors that cause changes ins mRNA expression upon Dicer loss. Through transcription factors over-expr

ORGANISM(S): Mus musculus

SUBMITTER: Sara Gosline 

PROVIDER: E-GEOD-61031 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress