H3K27ac ChIP in Dicer KO/WT [ChIP-seq]

ABSTRACT: We performed ChIP-Seq of H3K27ac in duplicate in both WT and KO mesenchymal stem cells to evaluate global transcriptional changes between the new cells. We identified putative transcription factor binding sites using GEM v1.1 in K27ac data as well as in p MicroRNAs (miRNAs) are small non-coding RNAs that regulates development and disease but induce only moderate repression of directs mRNA targets, suggesting that they coordinate with other modes ofs cellular regulation to effect large changes in gene expression. Ins this work we decouple direct effects of global miRNA loss froms transcriptional changes downstream in a pair of isogenic murines fibroblast cell lines with and without Dicer expression. Wes demonstrate how effects on direct miRNA targets are amplified bys transcription machiner

ORGANISM(S): Mus musculus

SUBMITTER: Sara Gosline 

PROVIDER: E-GEOD-61034 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress