Gene expression changes between sensitive and YK-4-279-resistant Ewing's Sarcoma cell lines

ABSTRACT: Microarray gene expression analysis conducted from cell lines in each of three cohorts: (1) Resistant ES cell lines, (2) Sensitive parental ES cell lines treated with YK-4-279 for 72 hours, and (3) untreated sensitive parental ES cell lines (Three replicates from TC32 & TC71 original parental cell lines) We used microarrays to detail the global programme of gene expression underlying mechanism of resistance to YK-4-279 within parental sensitive and resistant selected Ewing's Sarcoma cell lines. We identified distinct classes of up-regulated genes during this process. Total RNA was extracted from the cell lines using the Qiagen miRNeasy Mini kit. RNA quality was assessed to have an RNA integrity number before amplification and labeling using AffymetrixM-bM-^@M-^Ys GeneChip GeneChipM-BM-.3M-

ORGANISM(S): Homo sapiens

SUBMITTER: Joseph Ludwig 

PROVIDER: E-GEOD-61042 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress