Phenotypic responses of differentiated asthmatic human airway epithelial cultures to rhinovirus

ABSTRACT: We report the application of RNA sequencing technology for high-throughput profiling of gene expression responses to human rhinovirus infection at 24 hours in air-liquid interface human airway epithelial cell cultures derived from 6 asthmatic and 6 non-asthmatic donors. RNA-seq analysis identified sets of genes associated with asthma specific viral responses. These genes are related to inflammatory pathways, epithelial remodeling and cilium assembly and function, including those described previously (e.g. CCL5, CXCL10 and CX3CL1), and novel ones that were identified for the first time in this study (e.g. CCRL1, CDHR3). We concluded that air liquid interface cultured human airway epithelial cells challenged with live HRV are a useful in vitro model for the study of rhinovirus induced as

ORGANISM(S): Homo sapiens

SUBMITTER: Yongsheng Huang 

PROVIDER: E-GEOD-61141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress