Gene expression in E9.5 hearts comparing wt with Arid3b-null embryos
Ontology highlight
ABSTRACT: We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 hearts, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development. Hearts were dissected from E9.5 embryos (including the heart tube and the pharyngeal mesoderm and endoderm located beneath). Four wild-type and four mutant embryos were collected and pooled for each of the microarray triplicates.
Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 heads, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development. Heads were dissected from E9.5 embryos (including head and first branchial arch). Four wild-type and four mutant embryos were collected and pooled for each of the microarray triplicates.
Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 trunks, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development. Trunks were dissected from E9.5 embryos (including the body from the otic vesicle to somite 15 and forelimbs). Four wild-type and four mutant embryos were collected and pooled for each of the microarray triplicates.
Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b at E9.0 days of development, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development. Four wild-type and four mutant samples, consisting of 3-4 embryos each, were used.
Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 hearts, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development.
Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 trunks, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development.
Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 heads, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development.
Project description:Gene expression profiles were compared between wild-type and Jmjd3-/- embryos at E9.5 when mRNAs of posterior Hox genes are upregulated, because Jmjd3-/- mice embryos showed homeotic transformation. As expected, mRNAs of Hoxd10 and Hod11 were decreased in Jmjd3-/- embryos. Moreover, gene onthology (GO) analyses identified down-regulated genes (FC>1.5) in Jmjd3-/- embryos were M-bM-^@M-^\multicellular organismal developmentM-bM-^@M-^] and M-bM-^@M-^\developmental processM-bM-^@M-^]. Gene expressions in wild-type and Jmjd3-/- posterior half of embryos at E9.5 were examined. Two independent RNA samples of each genotypes were used to verify the reproducibility of the microarray analyses.
Project description:WT and Handsdown mutant ESC were differentatied into the meosdermal cell lineage and harvested at desiganted timepoints für differential expression profiling (n=3). Hearts of E9.5 mouse embryos were harvested and profiled to generate a represantative picture of RNAs (n=1). 4C-seq analysis of mESCs, cardiomyocytes and whole hearts from E11.5 mouse embryos
Project description:Understanding processes how the early stage kidney precursor gives rise to metanephric mesenchyme, which is a committed progenitor cells of adult kidney is important for the regeneration of kidney in vitro. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of global gene expression profiles of the developing kidney precursors. Those gene expression data provides insights into not only the stage specific marker genes but also the signals working in each population, which should be informative for the directed differentiation of pluripotent stem cells in vitro. Osr1-GFP knock-in mice were used to isolate kidney precursor cells from embryos at E8.5, E9.5 and E11.5. At E9.5 and E11.5 embryos, to identify the differences between nephron progenitors and surrounding mesenchyme, nephron progenitor populations were further enriched by gating Osr1-GFP positive Integrin alpha8 positive Pdgfr alpha negative population and compared with Osr1-GFP positive cells other than that gate. RNA was isolated from cells and the gene expression profiles were determined by microarrays.