HuR- dependent regulation of mRNA splicing is essential for the B cell antibody response [Ribo-Seq]

ABSTRACT: Post-transcriptional regulation of mRNA by the RNA binding protein HuR is required in B cells for the germinal centre reaction and for the production of class-switched antibodies in response to T-independent antigens. Transcriptome-wide examination of RNA isoforms, abundance and translation in HuR-deficient B cells, together with direct measurements of HuR-RNA interaction, revealed that HuR-dependent mRNA splicing affects hundreds of transcripts including the dihydrolipoyl succinyltransferase (Dlst), a subunit of the aketoglutaratedehydrogenase (aKGDH) enzyme. In the absence of HuR, defective mitochondrial metabolism results in high levels of reactive oxygen species and B cell death. Our study shows how post-transcriptional processes control the balance of energy metabolism required for B

ORGANISM(S): Mus musculus

SUBMITTER: Felix Krueger 

PROVIDER: E-GEOD-62134 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress