RNA-sequencing of mRNAs from control and CAP-D3 deficient Salmonella infected HT-29 cells

ABSTRACT: BACKGROUND & AIMS- More frequent interaction of bacteria with the colonic epithelium is associated with ulcerative colitis (UC). The identities of all proteins which promote bacterial clearance in colonic epithelial cells are unknown. Previously, we discovered that dCAP-D3 (Chromosome Associated Protein-D3), regulates responses to bacterial infection. We examined whether CAP-D3 promotes bacterial clearance in human colonic epithelium. METHODS- Clearance of Salmonella or adherent-invasive Escherichia coli LF82 was assessed by gentamycin protection assays in HT-29 and Caco-2 cells expressing CAP-D3 shRNA. CAP-D3 levels in colonic epithelial cells from healthy and UC patient tissues were analyzed by immunoblot. RNA-sequencing identified bacterially-induced CAP-D3 target genes. The role

ORGANISM(S): Homo sapiens

SUBMITTER: Michelle Longworth 

PROVIDER: E-GEOD-62520 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress