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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effect of spermine oxidase and p21 overexpression on skeletal muscle mRNA expression.

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Daniel Fox

PROVIDER: E-GEOD-63008 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Spermine oxidase maintains basal skeletal muscle gene expression and fiber size and is strongly repressed by conditions that cause skeletal muscle atrophy.

Bongers Kale S KS Fox Daniel K DK Kunkel Steven D SD Stebounova Larissa V LV Murry Daryl J DJ Pufall Miles A MA Ebert Scott M SM Dyle Michael C MC Bullard Steven A SA Dierdorff Jason M JM Adams Christopher M CM

American journal of physiology. Endocrinology and metabolism 20141118 2

Skeletal muscle atrophy is a common and debilitating condition that remains poorly understood at the molecular level. To better understand the mechanisms of muscle atrophy, we used mouse models to search for a skeletal muscle protein that helps to maintain muscle mass and is specifically lost during muscle atrophy. We discovered that diverse causes of muscle atrophy (limb immobilization, fasting, muscle denervation, and aging) strongly reduced expression of the enzyme spermine oxidase. Important ...[more]

PMID: 25406264

Similar Datasets

Effect of spermine oxidase overexpression on skeletal muscle mRNA expression.

Project description:For additional details see Bongers et al, Spermine Oxidase Maintains Basal Skeletal Muscle Gene Expression and Fiber Size, and Is Strongly Repressed by Conditions that Cause Skeletal Muscle Atrophy . Am J Physiol Endocrinol Metab. 2014 [under review] Bilateral tibialis anterior muscles of C57BL/6 mice were harvested seven days after transfection with spermine oxidase or control plasmid.

2014-12-04 | E-GEOD-63006 | biostudies-arrayexpress

Effect of p21 overexpression on skeletal muscle mRNA expression.

2014-12-04 | E-GEOD-63007 | biostudies-arrayexpress

p53 and ATF4 Mediate Distinct and Additive Mechanisms to Skeletal Muscle Atrophy During Limb Immobilization

Project description:p53 regulates a distinct subset of skeletal muscle mRNAs during immobilization-induced skeletal muscle atrophy For additional details see Fox et al, p53 and ATF4 mediate distinct and additive pathways to skeletal muscle atrophy during limb immobilization. Am J Physiol Endocrinol Metab. 2014 Aug 1;307(3):E245-61. Bilateral tibialis anterior muscles were harvested at three days for the following conditions: 1) hindlimb immobilization of C57BL/6 mice; 2) hindlimb immobilization of p53 mKO and littermate control mice; 3) transfection of wild type mice with p53 plasmid or control plasmid

2014-11-05 | E-GEOD-62897 | biostudies-arrayexpress

Dysregulated gene expression in Mef2a knockout mice subjected to cardiotoxin-induced muscle injury

Project description:We identified genes expressed in mouse skeletal muscle, during the process of muscle regeneration after injury, which are dysregulated in the absence of Mef2a expression. MEF2A is a member of the evolutionarily conserved MEF2 transcription factor family which has known roles in cardiac muscle development and function, but is not well studied in skeletal muscle. We performed a comparison of gene expression profiles in wild type and MEF2A knockout tibialis anterior muscle, seven days post-injury with cardiotoxin. The results indicated that a variety of genes expressed during muscle regeneration, predominantly microRNAs in the Gtl2-Dio3 locus, are dysregulated by the loss of MEF2A expression. Skeletal muscle RNA used in the present study included the following two sample groups: (WT) pooled total RNA from tibialis anterior muscle taken from 5 wild type mice at seven days post-injury with 10uM cardiotoxin; (KO) pooled total RNA from tibialis anterior muscle taken from 5 Mef2a knockout mice at seven days post-injury with 10uM cardiotoxin. All mice were between 2-4 months of age. Both male and female mice were used.

2013-02-12 | E-GEOD-41871 | biostudies-arrayexpress

Effect of denervation on mouse skeletal muscle mRNA levels.

Project description:Muscle denervation causes skeletal muscle atrophy. The goal of these studies was to determine the effects of denervation on skeletal muscle mRNA levels in C57BL/6 mice. For additional details see Ebert et al, Stress-Induced Skeletal Muscle Gadd45a Expression Reprograms Myonuclei and Causes Muscle Atrophy. JBC epub. June 12, 2012. Left sciatic nerves of C57BL/6 mice were transected. Seven days later bilateral tibialis anterior muscles were harvested. mRNA levels in denervated muscles were normalized to levels in contralateral innervated muscles.

2012-09-15 | E-GEOD-39195 | biostudies-arrayexpress

Gene expression profile of G93A-SOD1 mouse spinal cord, blood and muscle

Project description:To investigate the usefulness of gene expression as diagnostic biomarkers, we compared whole genome expression profiles of lumbar spinal cord with profiles of peripheral blood and tibialis anterior muscle in 16 mutant G93A-SOD1 mice and 15 wild type littermates. Total RNA obtained from blood, tibialis anterior muscle and lumbar spinal cord of G93A-SOD1 mice compared to wild type littermates.

2013-03-01 | E-GEOD-27933 | biostudies-arrayexpress

Differential gene expression analysis of follistain treated skeletal muscle

Project description:RNA-Seq analysis was performed to define the associated changes in gene expression of skeletal muscle treated with follistatin Skeletal muscle mRNA profiles from follistatin and control treated tibialis anterior muscles. Acute (3 day treatment, 3 control and 4 follistatin replicates) and chronic (7or 14 day treatment, 3 control and 4 follistatin replicates) timepoints were analysed.

2016-09-03 | E-GEOD-78965 | biostudies-arrayexpress

Effect of Smyd1 conditional knockout on gene expression in skeletal muscle

Project description:Transcriptome analysis by RNA-seq of tibialis anterior muscle from control and Smyd1 myocyte-specific conditional knockout mice at 6 weeks of age. Smyd1 is a methyltransferase specifically expressed in striated muscle and CD8+ T cells. Smyd1 deficiency resulted in centronuclear myopathy primarily affecting fast-twitch muscle fibers. These results provide insight into how loss of Smyd1 altered transcriptional programs resulting in centronuclear myopathy. 6 animals per group (control and Smyd1 conditional knockout)

2016-04-05 | E-GEOD-71679 | biostudies-arrayexpress

Expression data of satellite cells through muscle injury time course

Project description:Satellite cells are resident skeletal muscle stem cells responsible for muscle maintenance and repair. In resting muscle, satellite cells are maintained in a quiescent state. Satellite cell activation induces the myogenic commitment factor, MyoD, and cell cycle entry to facilitate transition to a population of proliferating myoblasts that eventually exit the cycle and regenerate muscle tissue. The molecular mechanism involved in the transition of a quiescent satellite cell to a transit-amplifying myoblast is poorly understood. We used microarrays to detail the global program of gene expression of in vivo satellite cell activation through muscle injury and identified RNA post-transcriptional regulation as a key component of satellite cell activation. Wild type or Sdc4-/- satellite cells were FACS isolated from resting muscle or from muscle 12h and 48h following barium chloride-induced muscle injury. 5000 cell equivalents of RNA was labeled and hybridized to MOE430v2 GeneChips (Affymetrix) and scanned as per manufacturers protocol. Probeset intensities were GCRMA normalized for further analysis including UPGMA hierarchical clustering, analysis of variance (ANOVA), and fold change.

2012-07-31 | E-GEOD-38870 | biostudies-arrayexpress

Profiling of glycerol- and CTX-induced models of muscle regeneration in mice

Project description:Utilizing glycerol and cardiotoxin (CTX) injections in the tibialis anterior muscles of M. musculus provides models of skeletal muscle damages followed by skeletal muscle regeneration. In particular, glycerol-induced muscle regeneration is known to be associated with ectopic adipogenesis. We characterized genome-wide expression profiles of tibialis anterior muscles from wild-type mice injured by either glycerol or CTX injection. Our goal was to detect gene expression changes during the time course of glycerol-induced and CTX-induced muscle regeneration models, that can lead to ectopic adipocyte accumulation. Tibialis anterior muscle of 12 week old wildtype C57BL/6J male mice injected i.m with either glycerol or CTX were collected 3, 7, 14 or 21 days after injection. 6 biological replicates were used for each time points. Please note that a few replicate samples did not pass QC test, thus, were removed from the submission. Unchallenged tibialis anterior muscles from a group of 5 wildtype C57BL/6J mice were also as a control. Total RNA was extracted, QCed and hybridized to Affymetrix microarrays.

2013-08-16 | E-GEOD-45577 | biostudies-arrayexpress

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