Contribution of cell-bound and cell-free cellulosomal versus non-cellulosomal systems in deconstruction of cellulosic biomass by Clostridium thermocellum

ABSTRACT: The high cellulose digestion capability of the anaerobic thermophilic bacterium, Clostridium thermocellum, can be attributed to its efficient glycoside hydrolase system, consisting of both a free-enzyme system and a cellulosomal system, wherein carbohydrate active enzymes (CAZymes) are organized by primary and secondary scaffoldin proteins into large protein complexes bound to the bacterial cell wall. Using an integrated series of experiments encompassing polysaccharide depolymerization assays, direct biochemical analyses and transcriptomics, we show herein that, in addition to cell-bound cellulosomes and M-bM-^@M-^\freeM-bM-^@M-^] enzymes, C. thermocellum naturally employs a system of cell-free cellulosomes that are not designed to be tethered to the cell and can diffuse away to attack

ORGANISM(S): Ruminiclostridium thermocellum DSM 1313

SUBMITTER: Charlotte Wilson 

PROVIDER: E-GEOD-63883 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress