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GLI1-regulated genes in human mammary epithelial cells and human breast cancer cells

ABSTRACT: To clarify transcriptional target genes of GLI1 in human mammary epithelial cells and breast cancer cells, primary culture cells of human mammary epithelium HMEC and breast cancer cell line MCF-7 were lentivirally transduced by either GLI1 or its control LacZ. Their RNA samples were served for expression analysis using AGILENT human 8x60K cDNA microarray. Array analysis of HMEC and MCF-7 expressing either GLI1 or LacZ. 1 color method was employed.

ORGANISM(S): Homo sapiens

SUBMITTER: Kenji Kasai

PROVIDER: E-GEOD-64350 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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GLI1-regulated genes in human mammary epithelial cells and human breast cancer cells

Project description:To clarify transcriptional target genes of GLI1 in human mammary epithelial cells and breast cancer cells, primary culture cells of human mammary epithelium HMEC and breast cancer cell line MCF-7 were lentivirally transduced by either GLI1 or its control LacZ. Their RNA samples were served for expression analysis using AGILENT human 8x60K cDNA microarray.

2015-04-28 | GSE64350 | GEO

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Project description:Increased expression of GLI1 is associated with poor prognosis for some breast cancer subtypes. A conditional transgenic GLI1 expressing mouse model, with or without heterozygous deletion of Trp53, was used to generate and study GLI1 induced mammary gland tumours. Tumour tissue was serially orthotopically transplanted for at least 10 generations in NSG mice.

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Downstream genes regulated by TSHZ2 in MCF-7 cells

Project description:To clarify downstream genes regulated by TSHZ2, MCF-7 cells were lentivirally transduced by TSHZ2 and its control LacZ and served their RNA samples for gene expression analysis using AGILENT human 8x60K cDNA microarray. Array analysis of MCF-7 cells expressing either TSHZ2 or LacZ. 1 color method was employed.

2016-01-11 | E-GEOD-64351 | biostudies-arrayexpress

Transcriptome study of human mammary epithelial cells following exposure to polymeric Eudragit RS nanoparticles part 1

Project description:To further study the transcriptome of human mammary epithelial cells (HMEC 184) after exposure to polymeric Eudragit RS nanoparticles (ENP), we investigate whole genome microarray expression to identify genes regulated by exposure or not to ENP. Changes in gene expression in HMEC 184 cells (50M-BM- % confluence) incubated with 25M-BM- M-BM-5g/mL ENPs (70M-BM- nm) for 24M-BM- h, and without incubation (control) were measured. Three biological replicates were performed as controls (US_01; US_02; US_04) and ENP exposed ones (US_6; US_07; US_08).

2013-08-01 | E-GEOD-45598 | biostudies-arrayexpress

Transcriptome study of human mammary epithelial cells following exposure to polymeric Eudragit RS nanoparticles part 2

Project description:To further study the transcriptome of human mammary epithelial cells (HMEC 184) after exposure to polymeric Eudragit RS nanoparticles (ENP), we investigate whole genome microarray expression to identify genes regulated by exposure or not to ENP. Changes in gene expression in HMEC 184 cells (90M-BM- % confluence) incubated with 25M-BM- M-BM-5g/mL ENPs (70M-BM- nm) for 24M-BM- h, and without incubation (control) were measured. Three biological replicates were performed as controls (US_09; US_10; US_11) and ENP exposed ones (US_12; US_13; US_14).

2013-08-01 | E-GEOD-45868 | biostudies-arrayexpress

Identification of GLI1 direct target genes in human pancreatic cancer cell line Panc-1

Project description:Panc-1GLI1ER and Panc-1ER were established from human pancreatic cancer cell line Panc-1. Panc-1GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, Panc-1ER harbor only EGFP-tagged AF2 domain. To identify GLI1 direct target genes, total RNAs were purified from the cells before and 3 hours after the beta-estradiol (E2) treatment. Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray. Keywords: Expression profiling by genome tiling array GLI1 direct target genes were identified in Panc-1 cells. Gene expression profiles of Panc-1GLI1ER and Panc-1ER cells were analyzed by AGILENT human 4x44 cDNA microarray before and 3 hours after treatment of beta-estradiol (E2) at doses of 10nM.

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2014-11-30 | GSE53729 | GEO

Changes in gene expression during acinar morphogenesis of normal-like human mammary epithelial cells in a 3D basement membrane cultures

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Gene expression profiling in MCF-7 cells exposed to blackcurrant extract.

Project description:Blackcurrants (Ribes nigrum L., Grossulariaceae) have a high content of anthocyanin polyphenols and these have been shown to have beneficial effects on health, owing to their antioxidant and anti-carcinogenic properties. This study analyzed the constituents of blackcurrant extract (BCE) and investigated its potential phytoestrogenic effects using a breast cancer cell line (MCF-7) overexpressing the estrogen receptor (ER) Î±. Microarray and ingenuity pathway analysis showed that BCE activated upstream genes such as ERÎ± and transforming growth factor beta 1, and upregulated the expression of many genes downstream of ERÎ±. MCF-7 cells were seeded in culture dish and maintain to confluent. Then medium replace with phenol-red-serum-free DMEM medium with or without BCE (50Î¼g/ml). After the cells were incubated for 24 h at 37 Â°C 5% CO2.

2014-06-10 | E-GEOD-58304 | biostudies-arrayexpress

Chromatin accessibility in response to knockdown of GLI1 and SMARCA2 in GLI1-mediated gene expression.

Project description:GLI1 is a transcription factor correlated to decreased survival in several cancers. We have identified SMARCA2 as a co-regulator that enhances GLI1-mediated transcriptional activity and functions through the C-terminal transcriptional activation domain of GLI1. Central domains including the ATPase motif of SMARCA2 physically interact with GLI1. Evaluation of DNA density indicates GLI1, like SMARCA2, can increase the DNA accessibility with a preference for sites distal to gene transcription start sites and outside the promoter regions (i.e. enhancers). The putative enhancers where accessibility is decreased by the knock down of GLI1 and SMARCA2 are located cis to genes, such as HHIP, that are regulated by GLI1 and implicated in cancer functions. At the putative enhancer for HHIP, the localization of SMARCA2 is at least partially dependent on GLI1’s presence. Understanding this transcriptional regulation by GLI1 and SMARCA2 through altering chromatin accessibility at enhances can provide additional therapeutic targets for cancers dependent on GLI1.

2020-05-11 | GSE143684 | GEO

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