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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Knockout mice lacking MG56

ABSTRACT: To survey altered gene expression in MG56-knockout muscle, total RNA preparations from mouse hindlimb muscle and hearts were subjected to gene microarray analysis. Results exhibit the effects of MG56 deficiency on overall transcription, and may further imply the biological role of MG56. Total RNA samples were preparated from Tibialis-anterior (TA) muscle and hearts of three P9 MG56-knockout mice and the corresponding wild-type mice were mixed and then subjected to Affymetrix Mus musculus microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Atsuhiko Ichimura

PROVIDER: E-GEOD-64868 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Knockout mice lacking MG56

Project description:To survey altered gene expression in MG56-knockout muscle, total RNA preparations from mouse hindlimb muscle and hearts were subjected to gene microarray analysis. Results exhibit the effects of MG56 deficiency on overall transcription, and may further imply the biological role of MG56.

2015-01-14 | GSE64868 | GEO

p53 and ATF4 Mediate Distinct and Additive Mechanisms to Skeletal Muscle Atrophy During Limb Immobilization

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2014-11-05 | E-GEOD-62897 | biostudies-arrayexpress

Global tibialis anterior skeletal muscle transcript data from male Carm1 skm-/- (HSAcre Tg/0 x Floxed Carm1) and Carm1 skm+/+ mice (HSAcre 0/0 x Floxed Carm1) on a chow diet.

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2022-08-24 | GSE189351 | GEO

Activation of the Aryl Hydrocarbon Receptor in Muscle Exacerbates Ischemic Pathology in Chronic Kidney Disease

Project description:This study examines the transcriptional profile at a single-nuclei resolution in mice with muscle-specific deletion of the aryl hydrocarbon receptor (AHR). Wildtype (AHRfl/fl, floxed Cre negative littermates) and AHRmKO (AHR muscle specific knockout) mice were given chronic kidney disease via adenine supplemented diet. Mice were then subjected to femoral artery ligation as an experimental model of peripheral artery disease. Tibialis anterior muscles were harvested at five days post-surgery and immediately snap frozen in liquid nitrogen and stored at -80C until nuclei isolation. In both libraries, muscle specimens were pooled from three males prior to nuclei isolations were performed.

2023-06-08 | GSE217751 | GEO

Dysregulated gene expression in Mef2a knockout mice subjected to cardiotoxin-induced muscle injury

Project description:We identified genes expressed in mouse skeletal muscle, during the process of muscle regeneration after injury, which are dysregulated in the absence of Mef2a expression. MEF2A is a member of the evolutionarily conserved MEF2 transcription factor family which has known roles in cardiac muscle development and function, but is not well studied in skeletal muscle. We performed a comparison of gene expression profiles in wild type and MEF2A knockout tibialis anterior muscle, seven days post-injury with cardiotoxin. The results indicated that a variety of genes expressed during muscle regeneration, predominantly microRNAs in the Gtl2-Dio3 locus, are dysregulated by the loss of MEF2A expression. Skeletal muscle RNA used in the present study included the following two sample groups: (WT) pooled total RNA from tibialis anterior muscle taken from 5 wild type mice at seven days post-injury with 10uM cardiotoxin; (KO) pooled total RNA from tibialis anterior muscle taken from 5 Mef2a knockout mice at seven days post-injury with 10uM cardiotoxin. All mice were between 2-4 months of age. Both male and female mice were used.

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Electrical pacing of mouse muscle

Project description:Mice were electrically stimulated by implanting a pacer. The hindlimb muscles received low frequency stimulation for three hours and the tibialis anterior muscle was harvested at different time points after the stimulation. Sham operated control mice were included in the experiment. mRNA was pooled from 6 mice at each time point and hybridized to the arrays together with mRNA from untreated mice (reference). Keywords: time-course

2004-12-24 | GSE1564 | GEO

RNA-seq of Mus musculus subjected to cecal ligation or puncture to investigate inflammation-induced skeletal muscle atrophy

Project description:C57Bl6 mice were subjected to cecal-ligation and puncture or sham surgery. Mice were sacrificed after 24 h or 96 h and muscle tissue was harvested. RNA from tibialis anterior muscle was isolated and subjected to RNA sequencing.

2022-12-31 | E-MTAB-10960 | biostudies-arrayexpress

Transcription profiling of mouse hearts from mutants lacking estrogen-related receptor alpha (ERRalpha), a nuclear receptor

Project description:Total RNA was isolated from 3 WT and 3 ERRalpha null hearts and independent hybridizations were performed using MOE430 2.0 microarrays. Expression profiling was conducted to determine changes in gene expression in hearts lacking ERRa. The expression of genes involved in heart and muscle development, muscle contraction, lipid metabolism, OxPhos, protein metabolism and transcription were affected by the loss of ERRa. Experiment Overall Design: 3 hearts from WT and 3 hearts from ERRalpha-null mice were used in the study. The expression of genes in the ERRalpha KO hearts were compared to the reference WT hearts.

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Analysis of skeletal muscle gene expression upon mouse hindlimb unloading and reloading

Project description:To investigate the molecular mechanisms governing the transition of skeletal muscle from atrophy to compensatory regeneration and hypertrophy, we employed a mouse model involving hindlimb unloading and subsequent reloading, conducting a comprehensive analysis of global gene expression using RNA-sequencing (RNA-seq). Gastrocnemius muscle samples were obtained from three groups: control mice, mice subjected to 10 days of hindlimb unloading-induced muscle atrophy, and mice reintroduced to normal cage activity for 1 day following the unloading period (reloading).

2023-07-16 | GSE237099 | GEO

Effects of spaceflight on murine skeletal muscle gene expression

Project description:Spaceflight results in a number of adaptations to skeletal muscle, including atrophy and shifts towards faster muscle fiber types. To identify changes in gene expression that may underlie these adaptations, microarray expression analysis was performed on gastrocnemius from mice flown on the STS-108 shuttle flight (11 days, 19 hours) versus mice maintained on earth for the same period. Additionally, to identify changes that were due to unloading and reloading, microarray analyses were conducted on calf muscle from ground-based mice subjected to hindlimb suspension (12 days) and mice subjected to hindlimb suspension plus a brief period of reloading (3.5 hours) to simulate the time between landing and sacrifice of the spaceflight mice.

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