Gene expression of M. acetivorans producing ANME-1 Mcr during growth on methane vs. growth on methanol

ABSTRACT: Our goal is to convert methane efficiently into liquid fuels that may be more readily transported. Since aerobic oxidation of methane is less efficient, we focused on anaerobic processes to capture methane, which are accomplished by anaerobic methanotrophic archaea (ANME) in consortia. However, no pure culture capable of oxidizing and growing on methane anaerobically has been isolated. In this study, Methanosarcina acetivorans, an archaeal methanogen, was metabolically engineered to take up methane, rather than to generate it. To capture methane, we cloned the DNA coding for the enzyme methyl-coenzyme M reductase (Mcr) from an unculturable archaeal organism from a Black Sea mat into M. acetivorans to effectively run methanogenesis in reverse. The engineered strain produces primarily acetat

ORGANISM(S): Methanosarcina acetivorans

SUBMITTER: Thomas Wood 

PROVIDER: E-GEOD-66445 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress