Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Enrichment of H3K9me2 on unsynapsed chromatin in C. elegans does not target de novo sites [ChIP-Seq]

ABSTRACT: Characterization of the H3K9me2 distribution in the C. elegans adult hermaphrodite genome using ChIP-Seq Examination of H3K9me2 in two different nematode strains: fer-1 control and fer-1;him-8 mutant, where the X chromosomes are unsynapsed. The fer-1 mutation prevents production of embryos.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Eleanor Maine 

PROVIDER: E-GEOD-67028 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Enrichment of H3K9me2 on Unsynapsed Chromatin in Caenorhabditis elegans Does Not Target de Novo Sites.

Guo Yiqing Y   Yang Bing B   Li Yini Y   Xu Xia X   Maine Eleanor M EM  

G3 (Bethesda, Md.) 20150708 9

Many organisms alter the chromatin state of unsynapsed chromosomes during meiotic prophase, a phenomenon hypothesized to function in maintaining germline integrity. In Caenorhabditis elegans, histone H3 lysine 9 dimethylation (H3K9me2) is detected by immunolabeling as enriched on unsynapsed meiotic chromosomes. Loss of the SET domain protein, MET-2, greatly reduces H3K9me2 abundance and results in germline mortality. Here, we used him-8 mutations to disable X chromosome synapsis and performed a  ...[more]

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