Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Specific Inflammatory Stimuli Lead to Distinct Platelet Responses in mice and Humans

ABSTRACT: Different inflammatory stimuli contribute to the formation of atherosclerosis. It is hypothesized that although the end result is the same - plaque formation in arterial vessels - the pathogenesis is dependent on the etiology. In particular, platelets will respond differently depending on the inflammatory stimuli and timepoint. Using a microarray and platelet inflammatory function studies, we identified the transcriptional and functional changes that occur early and late with different inflammatory stimuli. ApoE-/- C57BL/6 mice were left untreated (control) or administered oral P. gingivalis (Pg) or intranasal C. pneumoniae (Cp) for 3 weeks, and sacrificed either 1 day (early timepoint) or 9 weeks (late timepoint) after this 3-week period. A separate group of animals was fed a Western Diet (WD) for 9 weeks and then sacrificed. Whole blood samples were collected from each animal into citrate solution and serially centrifuged to produce a pure platelet population. RNA was extracted and pooled from each experimental group and hybridized to Affymetrix Mouse Gene 1.0 ST microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Adam Gower

PROVIDER: E-GEOD-68835 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Specific Inflammatory Stimuli Lead to Distinct Platelet Responses in Mice and Humans.

Beaulieu Lea M LM Clancy Lauren L Tanriverdi Kahraman K Benjamin Emelia J EJ Kramer Carolyn D CD Weinberg Ellen O EO He Xianbao X Mekasha Samrawit S Mick Eric E Ingalls Robin R RR Genco Caroline A CA Freedman Jane E JE

PloS one 20150706 7

<h4>Introduction</h4>Diverse and multi-factorial processes contribute to the progression of cardiovascular disease. These processes affect cells involved in the development of this disease in varying ways, ultimately leading to atherothrombosis. The goal of our study was to compare the differential effects of specific stimuli--two bacterial infections and a Western diet--on platelet responses in ApoE-/- mice, specifically examining inflammatory function and gene expression. Results from murine s ...[more]

PMID: 26148065

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Specific Inflammatory Stimuli Lead to Distinct Platelet Responses in mice and Humans

Publications

Specific Inflammatory Stimuli Lead to Distinct Platelet Responses in Mice and Humans.

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