BBr003: Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency

ABSTRACT: In mammalian preimplantation development, pluripotent cells are set aside from cells that contribute to extra-embryonic tissues. Although the pluripotent cell population of mouse and human embryos can be cultured as embryonic stem cells, little is known about the pathways involved in formation of a bovine pluripotent cell population, nor how to maintain these cells in vitro. The objective of this study was to determine the transcriptomic profile related to bovine pluripotency. Therefore, in vitro derived embryos were cultured in various culture media that recently have been reported capable of maintaining the naïve pluripotent state of human embryonic cells. Gene expression profiles of embryos cultured in these media were compared using microarray analysis and quantitative RT-PCR. Compare

ORGANISM(S): Bos taurus

SUBMITTER: Marian Groot Koerkamp 

PROVIDER: E-GEOD-69399 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress