Project description:The CrdR-ChIP profiling is comparing H. pylori gDNA without crdR interaction as control and CrdR interact with H. pylori gDNA (WT vs crdR). The goal was determine the crdR binding site on H. pylori genome, it provide possible crdR-regulated genes. 2 samples: H. pylori 26695 is control, and CrdR is experiment.
Project description:Methylated modifications of genome are common events in carcinogenesis and is involved in the tumorigenesis and progression of various cancers including gastric cancer Methylated DNA immunoprecipitation (MeDIP) combined with a human miRNA tiling microarray analysis demonstrated that there are much methylation differention between gastric cancers and adjacent controls microRNA gene methylation comparison of 3 pairs of gastric cancer and controls
Project description:Data for the third YPIC Challenge. A synthetic peptide was analyzed using HR-MS. The peptide contains a codeword/phrase/sentence. Can you decrypt it? Get your best mates and try to crack the challenge!
Project description:EGF is one of the most well-characterized growth factors and plays a crucial role in cell proliferation and differentiation. EGFR has been extensively explored as a therapeutic target against multiple types of cancers, such as lung cancer and glioblastoma. Recent studies have established a connection between deregulated EGF signaling and metabolic reprogramming, especially rewiring in aerobic glycolysis, which is also known as the Warburg effect and recognized as a hallmark in cancer. Pyruvate kinase M2 (PKM2) is a rate-limiting enzyme controlling the final step of glycolysis and serves as a major regulator of the Warburg effect. We previously showed that PKM2 T405/S406 O-GlcNAcylation, a critical mark important for PKM2 detetramerization and activity, was markedly upregulated by EGF. However, the mechanism by which EGF regulates PKM2 O-GlcNAcylation still remains uncharacterized. Here we demonstrated that EGF promoted O-GlcNAc transferase (OGT) binding to PKM2 by stimulating OGT Y976 phosphorylation. As a consequence, PKM2 O-GlcNAcylation and detetramerization were upregulated, leading to a significant decrease in PKM2 activity. Moreover, other than PKM2, the association of additional phosphotyrosine binding proteins, including STAT1, STAT3, STAT5, PKCδ and p85, which are reported factors bearing O-GlcNAcylation, with OGT was also enhanced when Y976 was phosphorylated. Together, EGF-dependent Y976 phosphorylation is critical for OGT-PKM2 interaction and we propose that this post-translational modification might be important for the substrate selection by OGT.
Project description:L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard<br>(University Aarhus, Denmark) and then self-propagated at the University of Seville.<br>Seeds were scarified and surface-sterilized,<br>germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture<br>of vermiculite and sand as solid support.<br>Five seedlings were planted in each pot and grown<br>during 35 days in a growth chamber under 16/8 hours<br>day/night, 20/18M-oM-?M-=C, with a photosynthetic photon flux<br>density of 250 M-oM-?M-=mol/m2M-oM-?M-=s and a constant humidity of 70%.<br> Plants were watered with Hornum nutrient solution.<br>Drought was applied withholding irrigation for the reported period<br>of time and sample plants or leaves were harvested for further analysis.
Project description:Introduction: Angong Niuhuang Wan (AGNHW), developed in the Qing dynasty (18th century) for treating consciousness disturbances caused by severe infections, has already been used to treat brain edema caused by ischemia-reperfusion. However, it remains unclear whether AGNHW can ameliorate vascular-origin brain edema caused by lipopolysaccharides (LPS).This study explores the ameliorative effects of AGNHW on cerebrovascular edema in mice caused by LPS, as well as its potential mechanisms.Mice were divided into four groups: Control, LPS 26 h, LPS 48 h, and LPS + AGNHW_M. Enriched phosphorylated peptides were extracted from mouse brain tissues at the corresponding time points and subjected to phosphoproteomic analysis.
Project description:This project is to investigate whether and how leucine affects endogenous OMM protein stability in C. elegans overexpressing cdc-48. Whole worm and enriched mitochondria samples (with/without leucine and/or CHX treatment) were measured.
Project description:L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard <br>(University Aarhus, Denmark) and then self-propagated at the University of Seville.<br> Ljgln2-2 mutant was isolated from photorespiratory mutant screening as described <br>previously (Orea et al., 2002; M�rquez et al., 2005; Betti et al., 2006). <br>The mutant progeny of two consecutive back-crosses with WT was used<br> for the present work. WT and mutant seeds were scarified and surface-sterilized,<br> germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture<br> of vermiculite and sand as solid support. <br>Five seedlings were planted in each pot and grown <br>during 35 days in a growth chamber under 16/8 hours<br> day/night, 20/18�C, with a photosynthetic photon flux <br>density of 250 �mol/m2�s and a constant humidity of 70%.<br> CO2 was automatically injected to a final concentration of 0.7% (v/v),<br> to allow for normal growth of Ljgln2-2 mutant in a photorespiration <br>suppressed atmosphere. Plants were watered with Hornum nutrient solution <br>(Handberg & Stougaard, 1992).<br> Drought was applied withholding irrigation for the reported period <br>of time and sample plants or leaves were harvested for further analysis.