DNA Cytosine Hydroxymethylation Levels Are Distinct Among Peripheral Blood Leukocytes

ABSTRACT: Peripheral blood leukocytes are the most commonly used surrogates to study epigenome-induced risk and epigenomic response to disease related stress. We considered the hypothesis that the TET enzyme catalyzed hydroxymethylation of 5mC to 5hmC might vary among peripheral blood leukocytes and reflect their responsiveness to environment. Reduction in TET1 and/or TET2 activity leads to the over-proliferation of various leukocyte precursors in bone marrow and acute leukemia, yet, the role of 5mC hydroxymethylation in peripheral blood is less well studied. We developed simplified protocols to rapidly and reiteratively isolate mostly non-overlapping leukocyte populations from a single small sample of fresh or frozen whole blood. Among peripheral leukocyte types we found extreme variation in the le

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Schmitz 

PROVIDER: E-GEOD-70519 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress