Transcriptomics,Genomics

Dataset Information

34

Profiling of CD14+ monocytes from paired rheumatoid arthritis (RA)-patient peripheral blood and synovial fluid samples


ABSTRACT: CD14+ monocytes sorted from the synovial fluid or peripheral blood of rheumatoid arthritis patients were analyzed by full transcriptome microarray analysis. Monocytes from healthy control samples (peripheral blood) were also profiled. Overall design: A total of 26 samples consisting of 16 paired and 2 un-paired synovial fluid and peripheral blood derived CD14+ monocytes from RA-patients, as well as peripheral blood CD14+ monocytes from 8 healthy control subjects were compared. Sorted monocytes (at least of 95% purity) from each of the donors were obtained individually, and compared by full transcriptome profiling.

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array [CDF: Brainarray ENSG Version 16]

SUBMITTER: Klaus Stensgaard Frederiksen 

PROVIDER: GSE71370 | GEO | 2017-01-30

SECONDARY ACCESSION(S): PRJNA291047

REPOSITORIES: GEO

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Publications

MicroRNA-155 contributes to enhanced resistance to apoptosis in monocytes from patients with rheumatoid arthritis.

Rajasekhar Megha M   Olsson Anton M AM   Steel Kathryn J A KJ   Georgouli Mirella M   Ranasinghe Ushan U   Brender Read Christine C   Frederiksen Klaus S KS   Taams Leonie S LS  

Journal of autoimmunity 20170122


Monocytes and macrophages are key mediators of inflammation in rheumatoid arthritis (RA). Their persistence at the inflammatory site is likely to contribute to immunopathology. We sought to characterise one mechanism by which persistence may be achieved: resistance to apoptosis and the role of mir-155 in this process. CD14+ monocytes from peripheral blood (PBM) and synovial fluid (SFM) of RA patients were found to be resistant to spontaneous apoptosis relative to PBM from healthy control (HC) in  ...[more]

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