Genome-wide analysis of the isogenic embryonic and induced pluripotent stem cell lines revealed no traces of parental cell type or reprogramming process and provided the approach for the best clone selection [methylation]

ABSTRACT: Genome wide DNA methylation profiling of two parental human embryonic stem cell (ESC) lines (hESM01 and n5), three n5-derived somatic cell lines (N-neurons, F-fibroblasts, R-retinal pigment epithelium), and three pairs of induced pluripotent stem cell (iPSC) clones (iN, iF, iR). All lines are isogenic. The Illumina Infinium 450k Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 450,000 CpGs. Bisulphite converted DNA from the 24 samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip. For each line there are a biological (different passages) or technical (same passage) replicate.

ORGANISM(S): Homo sapiens

SUBMITTER: Maria Shutova 

PROVIDER: E-GEOD-70737 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress