Transcription profiling of human fibroblasts synchronized and treated with 1.5 Gy irraditation to identify primary transcriptional regulation of cell-cycle-regulated genes upon DNA damage

ABSTRACT: The changes in global gene expression in response to DNA damage may derive from either direct induction or repression by transcriptional regulation or indirectly by synchronization of cells to specific cell cycle phases, such as G1 or G2. We developed a model that successfully estimated the expression levels of >400 cell-cycle-regulated genes in normal human fibroblasts based on the proportions of cells in each phase of the cell cycle. By isolating effects on the gene expression associated with the cell cycle phase redistribution after genotoxin treatment, the direct transcriptional target genes were distinguished from genes for which expression changed secondary to cell synchronization. Application of this model to ionizing radiation (IR)-treated normal human fibroblasts identified 150 o

ORGANISM(S): Homo sapiens

SUBMITTER: Tong Zhou 

PROVIDER: E-GEOD-7075 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress