RNAseq reveals different transcriptional profils of Klebsiella pneumoniae planktonic, sessile and biofilm-dispersed cells

ABSTRACT: Purpose: The goal of this study was to use RNA-seq to define the Klebsiella pneumoniae transcriptome recorded under 5 different experimental conditions, and to identify signature genes of each condition by comparing global transcriptional profiles. Methods: mRNA profiles were generated for Klebsiella pneumoniae CH1034 clinical isolate, in triplicate, by deep sequencing. Total RNAs were harvested from bacteria cultured at 37°C in M63B1 minimal media under different conditions: (i) planktonic aerobic condition at OD 620nm=0.250 (exponential growth-phase), (ii) overnight planktonic aerobic condition (stationnary growth-phase), (iii) biofilm in a flow-cell chamber after 7 hours of incubation (7-hours old biofilm), (iv) biofilm in a flow-cell chamber after 13 hours of incubation (13-hours old

ORGANISM(S): Klebsiella pneumoniae

SUBMITTER: Damien BALESTRINO 

PROVIDER: E-GEOD-71754 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress