Transcription profiling of chicken develomental time series of the retinal pigment epithelia

ABSTRACT: Purpose: The morphology of the RPE shows minimal change as the neural retina and choriocapillaris differentiate. Nonetheless, initial studies of barrier-related proteins suggest extensive remodeling of the RPE in response to this changing environment. A genomic approach was used to investigate the extent of this remodeling. Methods: RPE was isolated from E7, E10, E14 and E18 chick embryos and total RNA extracted for probing the entire genome on Affymetrix microarray chips. Statistical parameters using ANOVA were adjusted to yield a theoretical false discovery rate of 5%. STEM software was used to cluster genes into statistically related patterns of expression. Gene ontology clustering, using Affymetrix software was used for functional clustering. The proteinlounge.com database was used

ORGANISM(S): Gallus gallus

SUBMITTER: Lawrence Rizzolo 

PROVIDER: E-GEOD-7176 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress