Transcription profiling of human slow flow venous malformations and normal saphenous veins

ABSTRACT: Objective: Vascular malformations affect 3% of neonates. Venous malformations (VMs) are the largest group representing more than 50 % of cases. In hereditary forms of VMs gene mutations have been identified, but for the large group of spontaneous forms the primary cause and downstream dysregulated genes are unknown. Methods and Results: We have performed a global comparison of gene; expression in slow-flow VMs and normal saphenous veins using human whole genome micro-arrays. Genes of interest were validated with qRT-PCR. Gene expression in the tunica media was studied after laser micro-dissection of small pieces of tissue. Protein expression in endothelial cells (ECs) was studied with antibodies. We detected 511 genes more than 4-fold down- and 112 genes more than 4-fold up-regulated. Nota

ORGANISM(S): Homo sapiens

SUBMITTER: Lennart Opitz 

PROVIDER: E-GEOD-7190 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress