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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MRNA expression data for rat liver treated for 24h with RG3487 (180mg/kg po.) vs vehicle only (deionized water)

ABSTRACT: Gene expression analysis followed by gene onthology pathway analysis revealed that “cell cycle” and “growth and proliferation” pathways were significantly affected in the high dose group at the 24h time point. No consistent gene expression changes related to nuclear receptor activation, in particular target genes of CAR, PXR or PPAR were observed at the 24h time point Rat liver tissue was harvested 2h hours after treatment of rats with test compound or vehicle. Tissue was harvested in RNA-Later (Ambion Cat No 7021) for RNA extraction and hybridization to Affymetrix microarrays

ORGANISM(S): Rattus norvegicus

SUBMITTER: Laura Badi

PROVIDER: E-GEOD-72357 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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mRNA expression data for rat liver treated for 24h with RG3487 (180mg/kg po.) vs vehicle only (deionized water)

Project description:Gene expression analysis followed by gene onthology pathway analysis revealed that “cell cycle” and “growth and proliferation” pathways were significantly affected in the high dose group at the 24h time point. No consistent gene expression changes related to nuclear receptor activation, in particular target genes of CAR, PXR or PPAR were observed at the 24h time point

2015-08-26 | GSE72357 | GEO

TMEM203 is a Novel Regulator of Intracellular Calcium Homeostasis and is required for Spermatogenesis

Project description:Intracellular calcium signaling is critical for initiating and sustaining diverse cellular functions including transcription, synaptic signaling, muscle contraction, apoptosis and fertilization. Trans-membrane 203 (TMEM203) was identified here in cDNA overexpression screens for proteins capable of modulating intracellular calcium levels using activation of a calcium/calcineurin regulated transcription factor as an indicator. Overexpression of TMEM203 resulted in a reduction of Endoplasmic Reticulum (ER) calcium stores and elevation in basal cytoplasmic calcium levels. TMEM203 protein was localized to the ER and found associated with a number of ER proteins which regulate ER calcium entry and efflux. Mouse Embryonic Fibroblasts (MEFs) derived from Tmem203 deficient mice had reduced ER calcium stores and altered calcium homeostasis. Tmem203 deficient mice were viable though male knockout mice were infertile and exhibited a severe block in spermiogenesis and spermiation. Expression profiling studies showed significant alternations in expression of calcium channels and pumps in testes and concurrently Tmem203 deficient spermatocytes demonstrated significantly altered calcium handling. Thus Tmem203 is an evolutionarily conserved regulator of cellular calcium homeostasis, is required for spermatogenesis and provides a causal link between intracellular calcium regulation and spermiogenesis. Testes were harvested from control and Tmem203 null mice at 24 weeks of age. 4 wild type testes and 5 null mice testes were probed using Affymetrix Mouse Genome 430 2.0 platform.

2015-04-15 | E-GEOD-66791 | biostudies-arrayexpress

Transcriptional responses of control and MDV3100 resistant lines to DMSO or MDV3100

Project description:LNCaP-derived MDV3100-resistant clones were treated with MDV3100 for 24h prior to collection This experiment is designed to see if MDV3100 resistant clones remain responsive to MDV3100 Control and resistant clones were seeded in 6-well plates for 3d prior to treatment with DMSO or MDV3100 for 24h

2013-09-30 | E-GEOD-44927 | biostudies-arrayexpress

Testing rescue of AR signaling by ectopic expression of mutant AR allele in the presence of MDV-3100

Project description:Genetically engineered LNCaPs overexpressing various AR alleles were treated with 0.1% DMSO or 10uM MDV3100 for 24h prior to collection This experiment is designed to see if expressing the F876L/T877A mutant AR can rescue AR signaling in the presence of MDV3100 Engineered lines were seeded in 6-well plates for 3d with 100ng/ml doxycycline prior to treatment with 0.1% DMSO or 10uM MDV3100 for 24h

2013-09-30 | E-GEOD-44924 | biostudies-arrayexpress

Identification of androgen-regulated genes in RWPE-1-AR cells

Project description:Transcriptional profiling of RWPE-1 cells stably expressing human androgen receptor (as described in Altintas et al., Mol Cell Endocrinol 2011) treated with a non-metabolisable androgen, R1881 RWPE-1-AR cells were treated with R1881 during 3h or 24h and compared to control not treated cells. Three independent cell culture experiments for each treatment condition (vehicle or R1881 for 3h and 24h).

2011-05-12 | E-GEOD-29232 | biostudies-arrayexpress

Glutamine deprivation in U2OS cells

Project description:To understand the effects of glutamine deprivation on cell physiology we performed global analysis of gene expression in response to glutamine deprivation. U2OS cells were subjected to glutamine deprivation for 24h followed by RNA extraction and microarray analysis. U2OS cells were plated overnight followed by treatment for 24h with glutamine-containing and glutamine-depleted media. Three biological replicates were assayed for each condition.

2014-07-31 | E-GEOD-59931 | biostudies-arrayexpress

c-Jun protein expression in vivo induces systemic fibrosis in mice

Project description:Time course analysis of c-Jun expression at 24h resulted in upregulation of a number of well-known fibrogenesis-associated factors. We compared the global gene expression pattern of mouse whole bone marrow after c-Jun induction in vivo at 24h with no induction and applied to standard Affymetrix mouse arrays.

2016-07-26 | E-GEOD-84838 | biostudies-arrayexpress

Expression data from Arabidopsis seedlings treated with Pseudomonas aeruginosa (strain PA14)

Project description:Early stages of host microbe adaptations involve 'system status changes' (rewiring of pre-existing cellular signaling networks and components) of the host and microbe. We posited that under certain environmental conditions these changes leads to maladaptations and favor emergence of new infectious diseases, and these adaptations will have characteristic signatures representative of the adaptation. Here using Arabidopsis seedlings in a submerged environment treated with P. aerugionsa, we show one such rewired regulation where the master two-component regulator GacA (previously shown to act upstream of quorum sensing, including the regulator LasR, that in turn controls a subset of virulence factors) is completely dispensable. The gacA mutant behaves similar to wild type P. aeruginosa (strain PA14) by a number of read-outs. Consistent with that, the gene expression data here indicates that the transcriptome pattern of the host is identical when treated with wild type PA14 or PA14-gacA mutant. Single time point (10 day old Arabidopsis seedlings infected with wild type PA14 or mutant bacteria PA14DgacA, and analyzed 24h after infection) with two independent experimenal replicates per treatment

2011-01-22 | E-GEOD-26084 | biostudies-arrayexpress

Differential expression of E.coli mar/rob/soxS triple mutant and wild type in a mouse model of pyelonephritis

Project description:Mutation of marA, rob, and soxS causes a clinical strain of E.coli to be attenuated at d3 post-infection in a mouse model of pyelonephritis, here we extract RNA at d2 post infection to analyze transcriptional differences between the two strains. In order to purify enough bacterial RNA to perform microarray, we chose a time point (day 2 post-infection) just before the triple mutant begins to decrease in bacterial load. Mouse kidneys were extracted, homogonized, and samples were pooled in RNALater (Ambion) before extraction and analysis of the transcriptomes

2010-10-01 | E-GEOD-23417 | biostudies-arrayexpress

Transcription profiling by array of female Anopheles gambiae following control-PBS or sHz inoculation in order to determine which genes and pathways were activated

Project description:Female mosquitoes, Anopheles gambiae, were injected with control-PBS or 200micrograms per ml hemozoin (sHz). Fat bodies were collected 24h post injection, before feeding. RNA was extracted and cDNA synthesized for microarray experiment. Three independent experiments (3x control and 3x sHz) arrays were performed. Fold change of genes expression in sHz-treated mosquitoes compared to PBS-treated ones was calculated. We aimed to describe how hemozoin activates the mosquito's immune system and which pathways are involved.

2016-04-08 | E-MTAB-1072 | biostudies-arrayexpress

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