Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

9 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data of uropathogenic E. coli CFT073 after exposure to subinhibitory concentrations of five biocides.

ABSTRACT: Biocides are chemical compounds widely used in hospital settings for a variety of purposes, but mainly for disinfection. The chemical properties of a biocide, as well as the biocide concentration, influence which cellular targets are affected. Exposure of bacteria to residual concentrations of biocides could lead to development of increased resistance towards the biocide in use, as well as cross-resistance towards other antimicrobials, including antibiotics. The aim of this study was to examine whether biocides could induce any potentially relevant genes that could affect pathogen's drug resistance or fitness. By examining global gene expression of the uropathogenic Escherichia coli CFT073 after exposure to subinhibitory concentrations of five biocides (benzalkonium chloride - BAC, chlorhexidine - CHX, ethanol - EtOH, hydrogen peroxide - H2O2, triclosan - TSN), we found that each biocide changed expression of different groups of genes and that exposure to ethanol caused changes in expression of the largest number of genes among all biocides. In general, the five biocides tested in this study at subinhibitory concentrations did not increase the resistance potential of the pathogen to other antimicrobials. We could, however, identify clusters of genes that could possibly help the strain to grow in the presence of a biocide in the medium. A culture of E. coli CFT073 without any biocide treatment served as the control sample. That culture was grown under the exact same conditions as the five biocide-treated samples. Each sample was collected in three biological replicates at the mid-exponential phase of growth.

ORGANISM(S): Escherichia coli

SUBMITTER: Malgorzata Ligowska-Marzeta

PROVIDER: E-GEOD-72738 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Comparison of gene expression profiles of uropathogenic Escherichia coli CFT073 after prolonged exposure to subinhibitory concentrations of different biocides

Project description:Biocides are chemical compounds widely used in hospital settings for a variety of purposes, but mainly for disinfection. The chemical properties of a biocide, as well as the biocide concentration, influence which cellular targets are affected. Exposure of bacteria to residual concentrations of biocides could lead to development of increased resistance towards the biocide in use, as well as cross-resistance towards other antimicrobials, including antibiotics. The aim of this study was to examine whether biocides could induce any potentially relevant genes that could affect pathogen's drug resistance or fitness. By examining global gene expression of the uropathogenic Escherichia coli CFT073 after exposure to subinhibitory concentrations of four biocides (benzalkonium chloride - BAC, chlorhexidine - CHX, hydrogen peroxide - H2O2, triclosan - TSN), we found that each biocide changed expression of different groups of genes and that exposure to benzalkonum chloride caused changes in expression of the largest number of genes among all biocides. In general, the four biocides tested in this study at subinhibitory concentrations did not increase the resistance potential of the pathogen to other antimicrobials. We could, however, identify clusters of genes that could possibly help the strain to grow in the presence of a biocide in the medium.

2019-12-05 | GSE135556 | GEO

Comparison of gene expression profiles of uropathogenic Escherichia coli CFT073 after prolonged exposure to subinhibitory concentrations of different biocides

Project description:Comparison of gene expression profiles of uropathogenic Escherichia coli CFT073 after prolonged exposure to subinhibitory concentrations of different biocides

| PRJNA559278 | ENA

Induction of virulence genes in Listeria monocytogenes serotype 4b upon exposure to concentrations of biocides

Project description:Study on genome-wide expression in L.monocytogenes str4b F2365 exposed to five different biocides at three different concentrations

2010-11-27 | GSE16336 | GEO

Pseudomonas fluorescens transcriptome in flowback water

Project description:The goal of the study was to examine the influence of flowback water exposure on bacterial survival and biocide tolerance. The transcriptome of P. fluorescens was analyzed using RNA-seq to determine the gene rxpression profile changes occuring upon flowback water exposure. The results indicate that that P. fluorescens induces a well-coordinated genetic response that aids in its survival in flowback water as well as imparts enhanced tolerance against typically used slow acting biocides such as gluteraldehyde but increased susceptibity towards sodium hypochlorite. RNA-seq data demonstrated significant induction of genes involved in osmotic stress, energy production and conversion, membrane integrity, protein transport among others.

2014-12-23 | GSE55388 | GEO

Gene expression profiling of Salmonella enterica serovar Typhimurium exposed to subinhibitory concentrations of fosmidomycin and kanamycin

Project description:Expression profiling on a total of 4,391 genes was conducted to identify regulated S. typhimurium genes upon exposure to subinhibitory concentrations of the MEP pathway antibiotic fosmidomycin. These results were subsequently compared to a similar gene expression analysis of subinhibitory exposures of S. typhimurium to the bactericide kanamycin

2016-08-28 | GSE50480 | GEO

Impact of sulfamethoxazole on a riverine microbiome

Project description:Freshwater environments such as rivers receive effluent discharges from wastewater treatment plants, representing a potential hotspot for antibiotic resistance genes (ARGs). These effluents also contain low levels of different antimicrobials including biocides and antibiotics such as sulfonamides that can be frequently detected in rivers. The impact of such exposure on ARG prevalence and microbial diversity of riverine environment is unknown, so the aim of this study was to investigate the release of a sub-lethal concentration (<4 g L-1) of the sulfonamide compound sulfamethoxazole (SMX) on the river bacterial microbiome using a microflume system. This system was a semi-natural in-vitro microflume using river water (30 L) and sediment, with circulation to mimic river flow. A combination of ‘omics’ approaches were conducted to study the impact of SMX exposure on the microbiomes within the microflumes. Metaproteomics did not show differences in ARGs expression with SMX exposure in water.

2021-07-06 | PXD023822 | Pride

Pseudomonas fluorescens transcriptome in flowback water

2014-12-23 | E-GEOD-55388 | biostudies-arrayexpress

Induction of virulence genes in Listeria monocytogenes serotype 4b upon exposure to concentrations of biocides

Project description:Study on genome-wide expression in L.monocytogenes str4b F2365 exposed to five different biocides at three different concentrations The study was carried out with a total of 34 microarrays using total RNA recovered from L.monocytogenes str4b F2365, which has been exposed for 1 hour (t =1) to five different biocides at three different concentrations (sub-inhibitory as well as inhibitory) in two-fold (30 exps). As reference samples, RNA of untreated cells were used in two-fold directly after pre-culturing (t=0) and after one hour of preculturing (t=1 hour; 4 exps). Each microarray read out includes expression levels of 2,821 genes from L.monocytogenes str4b F2365in two-fold, with a total of twelve 60-mer probe pairs (PM/MM) per gene.

2010-11-27 | E-GEOD-16336 | biostudies-arrayexpress

Global cellular adaptation to biocides and antibiotics in Salmonella enterica

Project description:The present project investigates transcriptomics changes in laboratory mutants of Salmonella typhimurium SL1344 obtained by pre-exposure to biocides, including triclosan (TRI), benzalkonium chloride (BZC), and chlorexidine (CHX), and antibiotics including Ampicillin (AP) and ciprofloxacin (Cip), as well as in natural isolates selected for their resistance to these same biocides. Changes in gene expression were investigated using a 12k combimatrix customarray, design-based on the genome of SL1344 as well as a variety of genes of plasmid origins.

2016-06-30 | E-MTAB-2554 | biostudies-arrayexpress

Downregulation of autolysin-encoding genes by phage-derived lytic proteins inhibits biofilm formation in Staphylococcus aureus

Project description:Phage-derived lytic proteins are a promising alternative to conventional antimicrobials. One of their most interesting properties is that they do not readily select for resistant strains, which is likely due to the fact that their targets are essential for the viability of the bacterial cell. Moreover, genetic engineering allows the design of new "tailor-made" proteins that may exhibit improved antibacterial properties. One example of this is the chimeric protein CHAPSH3b, which consists of a catalytic domain from the virion-associated peptidoglycan (PG) hydrolase of phage vB_SauS-phiIPLA88 (HydH5) and the cell wall binding domain of lysostaphin. CHAPSH3b had previously shown the ability to kill S. aureus cells. Here, we demonstrate that this lytic protein also has potential for the control of biofilm-embedded S. aureus cells. Additionally, subinhibitory doses of CHAPSH3b can decrease biofilm formation by some S. aureus strains. Transcriptional analysis revealed that exposure of S. aureus cells to this enzyme leads to the downregulation of several genes coding for bacterial autolysins. One of these proteins, namely the major autolysin AtlA, is known to participate in staphylococcal biofilm development. Interestingly, an atl mutant strain did not display inhibition of biofilm development when grown at subinhibitory concentrations of CHAPSH3b, contrary to the observations made for the parental and complemented strains. Also, deletion of atl led to low-level resistance to CHAPSH3b and endolysin LysH5. Overall, our results reveal new aspects that should be considered when designing new phage-derived lytic proteins aimed for antimicrobial applications.

2017-03-16 | GSE94512 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data