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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Indentification samll RNA loci that are synthesized by RNA-dependent RNA polymerases1/2/6

ABSTRACT: To identify RDR1/2/6 dependent small RNA loci of transcribed regions under 2hr drought stress- and untreated conditions, expression profiles of small RNA between rdr1/2/6 and wild-type using hi-seq 2000. rdr1/2/6 and wild-type plants (Arabidopsis thaliana ecotype Columbia) were grown on MS medium for 2weeks. Then, their plants were transfered to dry plastic dish for 2 hours. small RNA fraction was s extracted from the sample using Ambion mirVana miRNA Isolation Kit. small RNA labrary was constructed using TruSeq Small RNA Library Preparation Kits and applied to hi-seq 2000.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Akihiro Matsui

PROVIDER: E-GEOD-72982 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of small RNA loci that are synthesized by RNA-dependent RNA polymerases1/2/6

Project description:To identify RDR1/2/6 dependent small RNA loci of transcribed regions under 2hr drought stress- and untreated conditions, expression profiles of small RNA between rdr1/2/6 and wild-type using hi-seq 2000.

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