Bisulfite Sequencing for Studying 5mC DNA Methylation Distribution in Vibrio Cholerae

ABSTRACT: DNA methylation is a key epigenetic regulator in all domains of life, yet the effects of most bacterial DNA methyltransferases on cellular processes are largely undefined. Here, we used diverse techniques, including bisulfite sequencing, transcriptomics, and transposon insertion site sequencing to extensively characterize a 5-methylcytosine (5mC) methyltransferase, VchM, in the cholera pathogen, Vibrio cholerae. We have comprehensively defined VchM's DNA targets, its genetic interactions and the gene networks that it regulates. Although VchM is a relatively new component of the V. cholerae genome, it is required for optimal V. cholerae growth in vitro and during infection. Unexpectedly, the usually essential σE cell envelope stress pathway is dispensable in ΔvchM V. cholerae, likely due

ORGANISM(S): Vibrio cholerae

SUBMITTER: Shijia Zhu 

PROVIDER: E-GEOD-73974 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress