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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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DCAP-D3 ChIP-seq in Drosophila S2 cells

ABSTRACT: The goal of this experiment was to identify genomic dCAP-D3 binding sites in Drosophila S2R+ cells dCAP-D3 was immunoprecipitated in two separate experiments with antibody YZ834 which was developed in the Longworth lab. IgG immunoprecipiation was performed alongside the dCAP-D3 immunoprecipitation to serve as controls for each experiment. Input chromatin was also harvested from each of the two experiments.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Michelle Longworth

PROVIDER: E-GEOD-74413 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Drosophila Condensin II subunit Chromosome-associated protein D3 regulates cell fate determination through non-cell-autonomous signaling.

Klebanow Lindsey R LR Peshel Emanuela C EC Schuster Andrew T AT De Kuntal K Sarvepalli Kavitha K Lemieux Madeleine E ME Lenoir Jessica J JJ Moore Adrian W AW McDonald Jocelyn A JA Longworth Michelle S MS

Development (Cambridge, England) 20160617 15

The pattern of the Drosophila melanogaster adult wing is heavily influenced by the expression of proteins that dictate cell fate decisions between intervein and vein during development. dSRF (Blistered) expression in specific regions of the larval wing disc promotes intervein cell fate, whereas EGFR activity promotes vein cell fate. Here, we report that the chromatin-organizing protein CAP-D3 acts to dampen dSRF levels at the anterior/posterior boundary in the larval wing disc, promoting differe ...[more]

PMID: 27317808

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