UV-irradiation of skin enhances glycolytic flux in bone marrow-differentiated dendritic cells

ABSTRACT: Following UV irradiation of skin, dendritic cells (DCs) differentiating from the bone marrow (BM) of mice have a reduced ability to prime new immune responses; their reduced immunogenicity is maintained for at least 16 weeks in UV-chimeric mice. We hypothesized that different metabolic states underpin changes in DC function. Compared with DCs from the BM of non-irradiated mice, DCs from the BM of UV-irradiated mice produced more lactate and utilized greater amounts of glucose, a profile that was supported by greater glycolytic flux when incubated in low-serum-containing medium. Responses to a mitochondrial stress test were similar suggesting that the DCs from the BM of UV-irradiated mice had not switched from a profile of oxidative phosphorylation, but were imprinted for greater glycolytic

ORGANISM(S): Mus musculus

SUBMITTER: Kim Carter 

PROVIDER: E-GEOD-74468 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress