Transcriptome analysis of the C. glutamicum deletion mutant of rnc, which encodes an RNase III homolog

ABSTRACT: The Corynebacterium glutamicum R cgR_1959 gene encodes an endoribonuclease of the RNase III family. Deletion mutant of cgR_1959 (Δrnc mutant) showed an elongated cell shape, and presence of several lines on the cell surface, indicating a required of RNase III for maintaining normal cell morphology in C. glutamicum. The level of mraZ mRNA was increased, whereas cgR_1596 mRNA encoding a putative cell wall hydrolase and ftsEX mRNA were decreased in the Δrnc mutant. The half-life of mraZ mRNA was significantly prolonged in the Δrnc and the Δpnp mutant strains. This indicated that the degradation of mraZ mRNA was performed by RNase III and the 3′-to-5′ exoribonuclease, PNPase. Northern hybridization and primer extension analysis revealed that the cleavage site for mraZ mRNA by RNase II

ORGANISM(S): Corynebacterium glutamicum

SUBMITTER: Tomoya Maeda 

PROVIDER: E-GEOD-75080 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress