MicroRNA-offset RNA regulates gene expression and cell proliferation (Small RNA-Seq)

ABSTRACT: MicroRNA-offset RNAs (moRs) were first identified in simple chordates and subsequently in mouse and human cells by deep sequencing of short RNAs. MoRs are derived from sequences located immediately adjacent to microRNAs (miRs) in the primary miR (pri-miR). Currently moRs are considered to be simply a by-product of miR biosynthesis that lack biological activity. Here we show for the first time that a moR is biologically active. We now demonstrate that endogenous and over-expressed moR-21 significantly alters gene expression and inhibits the proliferation of vascular smooth muscle cells (VSMC). We report that the "seed region" of moR-21 as well as the "seed match region" in the target gene 3'UTR are indispensable for moR-21-mediated gene down-regulation. We further demons

ORGANISM(S): Mus musculus

SUBMITTER: Gavin Schnitzler 

PROVIDER: E-GEOD-75113 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress