Comparative genomic hybridizations of Mycobacterium avium isolates obtained from multiple host species

ABSTRACT: A comparative genomic approach was used to identify large sequence polymorphisms among Mycobacterium avium isolates obtained from a variety of host species. DNA microarrays were used as a platform for comparing mycobacteria field isolates with the sequenced bovine isolate Mycobacterium avium subsp. paratuberculosis (Map) K10. ORFs were classified as present or divergent based on the relative fluorescent intensities of the experimental samples compared to Map K10 DNA. Map isolates cultured from cattle, bison, sheep, goat, avian, and human sources were hybridized to the Map microarray. Three large deletions were observed in the genomes of four Map isolates obtained from sheep and four clusters of ORFs homologous to sequences in the Mycobacterium avium subsp. avium (Maa) 104 genome were i

ORGANISM(S): Mycobacterium avium

SUBMITTER: Srinand Sreevatsan 

PROVIDER: E-GEOD-7622 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress