Transcription profiling of human amnion epithelial FL cells treated with benzo(a)pyrene diol epoxide

ABSTRACT: Genotoxic agents cause cellular DNA damage and stress responses, including transcriptional changes. Here we focused on the early transcriptional responses of human cells to benzo(a)pyrene diol epoxide (BPDE), which causes bulky DNA adduct damage. Human amnion epithelial FL cells were exposed to three doses of BPDE (5, 50, and 500 nM) and the vehicle control DMSO, and differential gene expression profiles were obtained 4 h after exposure using oligonucleotide microarrays followed by validation with quantitative real-time RT-PCR. Compared with a few genes affected by the low and medium-dose exposure, extensive and robust changes in gene expression were induced by the high-dose BPDE. We found that the expression of cell cycle-regulators, signaling molecules and transcription factors were sign

ORGANISM(S): Homo sapiens

SUBMITTER: Lu XiangYun 

PROVIDER: E-GEOD-7675 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress