Transcriptomics,Genomics

Dataset Information

43

Transcriptional response of normal human lung WI-38 fibroblasts to benzo[a]pyrene diol epoxide: a dose-response study


ABSTRACT: Cellular responses to carcinogens are typically studied in transformed cell lines, which do not reflect the physiological status of normal tissues. To address this question, we have characterized the transcriptional program and cellular responses of normal human lung WI-38 fibroblasts upon exposure to the ultimate carcinogen benzo[a]pyrene diol epoxide (BPDE). Exposure to BPDE induces a strong inflammatory response in WI-38 primary fibroblasts. Whole-genome microarray analysis shows induction of several genes related to the production of inflammatory factors, including those that encode interleukins (ILs), growth factors, and enzymes related to prostaglandin synthesis and signaling. This is the first demonstration that a strong inflammatory response is triggered in primary fibroblasts in response to a reactive diol epoxide derived from a polycyclic aromatic hydrocarbon. Overall design: Normal human lung WI-38 fibroblasts were exposed to vehicle control (DMSO) and increasing doses (0.1, 0.5 and 1 uM) of anti-BPDE, respectively. The genome wide transcriptional response of the three treatments were compared to that of the control, respectively, in quadruplicates (Control and 1 uM) and triplicates (0.1 and 0.5 uM).

REANALYSED by: GSE86362GSE119128

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Kristian Dreij   

PROVIDER: GSE19510 | GEO | 2010-01-01

SECONDARY ACCESSION(S): PRJNA122331

REPOSITORIES: GEO

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Publications

Benzo[a]pyrene diol epoxide stimulates an inflammatory response in normal human lung fibroblasts through a p53 and JNK mediated pathway.

Dreij Kristian K   Rhrissorrakrai Kahn K   Gunsalus Kristin C KC   Geacintov Nicholas E NE   Scicchitano David A DA  

Carcinogenesis 20100409 6


Cellular responses to carcinogens are typically studied in transformed cell lines, which do not reflect the physiological status of normal tissues. To address this question, we have characterized the transcriptional program and cellular responses of human lung WI-38 fibroblasts upon exposure to the ultimate carcinogen benzo[a]pyrene diol epoxide (BPDE). In contrast to observations in cell lines, we find that BPDE treatment induces a strong inflammatory response in these normal fibroblasts. Whole  ...[more]

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