Transcription profiling of human MDA-MB-453 breast cancer cell lines treated with actein to assess effects on the growth as a function of time and concentration.

ABSTRACT: Previous studies indicate that the triterpene glycoside actein from the herb black cohosh inhibits growth of human breast cancer cells. This study seeks to identify genes altered in human breast cancer cells by treatment with actein, using gene expression analysis. We treated MDA-MB-453 human breast cancer cells with actein at 2 doses, 20 or 40 μg/mL, for 6 or 24 h. We identified 5 genes that were activated after each of the treatments that are known to play a role in cellular responses to diverse stresses, including the DNA damage and unfolded protein responses. In addition, four genes that mediate the integrated stress response (ISR), including activating transcription factor 4, were induced under at least one of the 4 treatment conditions. We used hierarchical clustering to define clus

ORGANISM(S): Homo sapiens

SUBMITTER: Linda Saxe Einbond 

PROVIDER: E-GEOD-7848 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress