Genome-wide chromatin landscape of chemical-induced neural stem cell reprogramming from defined fibroblasts

ABSTRACT: Cellular reprogramming using chemically defined conditions, without genetic manipulation, is a promising approach for generating clinically relevant cell types for regenerative medicine and drug discovery. However, small molecule-driven approaches for inducing lineage-specific stem cells from somatic cells across lineage boundaries have been challenging to develop. Here, we report highly efficient reprogramming of mouse fibroblasts into induced neural stem cell-like cells (ciNSLCs) using a cocktail of nine small molecules (M9). The resulting ciNSLCs closely resemble primary neural stem cells molecularly and functionally. Transcriptome analysis revealed that M9 induces a gradual and specific conversion of fibroblasts towards a neural fate. During reprogramming specific transcription factors

ORGANISM(S): Mus musculus

SUBMITTER: Sheng Ding 

PROVIDER: E-GEOD-78977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress