Staufen Negatively Modulates MicroRNA Activity in Caenorhabditis elegans

ABSTRACT: The double-stranded RNA-binding protein Staufen has been implicated in various posttranscriptional gene regulatory processes. Here, we demonstrate that the Caenorhabditis elegans homolog of Staufen, STAU-1, functionally interacts with microRNAs. Loss-of-function mutations of stau-1 significantly suppress phenotypes of let-7 family microRNA mutants, a hypomorphic allele of dicer and a lsy-6 microRNA partial loss-of-function mutant. Furthermore, STAU-1 modulates the activity of lin-14, a target of lin-4 and let-7 family microRNAs, and this modulation is abolished when the 3' untranslated region of lin-14 is removed. Deep sequencing of small RNA cDNA libraries reveals no dramatic change in the levels of microRNAs, or other small RNA populations between wild-type and stau-1 mutants, with the

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Isana Veksler-Lublinsky 

PROVIDER: E-GEOD-79217 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress