Transcriptome analysis of Escherichia coli during dGTP Starvation

ABSTRACT: Our laboratory has recently discovered that E. coli cells starved for the DNA precursor dGTP are killed efficiently (dGTP starvation) in a manner similar to that described for Thymineless Death (TLD). Conditions for specific dGTP starvation can be achieved by depriving an E. coli optA1 gpt strain of the purine nucleotide precursor hypoxanthine (Hx). To gain insight into the mechanisms underlying dGTP starvation, we conducted genome-wide gene expression analyses on actively growing optA1 gpt strains subjected to hypoxanthine deprivation for increasing periods of time. The data show that, upon Hx withdrawal, the optA1 gpt strain displays a diminished ability to de-repress the de novo purine biosynthesis genes, and this is likely due to internal guanine accumulation. The impairment to ful

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: NIEHS Microarray Core 

PROVIDER: E-GEOD-80002 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress