Transcription profiling of Drosophila larval salivary glands of late Drosophila prepupae expressing GAL4

ABSTRACT: The yeast transcription factor GAL4 has been reported to cause cell death and to have other biological effects when expressed in Drosophila (Kramer and Staveley, 2003: Genet. Mol. Res. 2, 43; Rezaval et al., 2007: Eur. J. Neurosci. 25, 683). Using heat-shock-induced expression of GAL4 to drive expression of a UAS-senseless responder gene in transcriptional profiling experiments, we found that the underlying cause of these effects might be a genomic response to GAL4. To further characterize this response and to account for GAL4-independent changes caused by the transgene integration, GAL4 was expressed from two copies of the transgene in two independent lines, P{GAL4-Hsp70.PB}89-2-1 (short P{hs-GAL4}89) and P{hs-GAL4}X1. In addition, GAL4 was expressed from only one copy of the transgene in

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Michael Lehmann 

PROVIDER: E-GEOD-8620 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress