Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting

ABSTRACT: The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres, where it provides the cohesive counter-force to bi-polar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences transiently split in pre-anaphase cells, in some organisms up to several micrometeres. This M-bM-^@M-^Xcentromere breathingM-bM-^@M-^Y presents a paradox, how sister sequences separate where cohesin is most enriched. We now show that in the budding yeast S. cerevisiae, cohesin binding diminishes over centromeric sequences that split during breathing. We see no evidence for cohesin translocation to surrounding sequences, suggesting that cohesin is removed from centromeres during breathing. Two pools

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Maria Ocampo-Hafalla 

PROVIDER: E-GEOD-8661 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress