Budding yeast cohesin (Mcd1) distribution in mitotically arrested cells

ABSTRACT: genomic localization of the budding yeast cohesin complex was mapped in mitotically arrested cells by Mcd1 ChIP followed by hybridization to high-density tiled microarrays Cells were synchronized first in G1 and then released into media containing the microtubule poison nocodazole. Cells were fixed and processed for ChIP once arrested as large-budded cells. Immunoprecipitated DNA and total genomic DNA not subjected to immunoprecipitation were competitively hybridized to Nimblegen whole genome arrays.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Paul Megee 

PROVIDER: E-GEOD-18021 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress