Comprehensive gene expression analysis of Bruce-4 embryonic stem cells by SAGE

ABSTRACT: Mouse embryonic stem cells lines derived from different mouse strains show considerable variation concerning their culture conditions, gene targeting efficiency and germ line transmission capacity. To gain insight into the basis for this phenomenon, we generated a SAGE library of the C57BL/6 derived ES cell line Bruce-4 and compared their gene expression to 129 strain-derived cells, which in general show the best performance in gene targeting experiments. Gene Ontology (GO) analyses for functional groups of genes demonstrated considerably higher expression of clusters indicating cellular differentiation as well as cell-cell and cell-matrix adhesion in R1 than in Bruce-4 cells. At the single gene level, almost 90% of the most strongly expressed transcripts in each library were significantly

ORGANISM(S): Mus musculus

SUBMITTER: Sandra Pennartz 

PROVIDER: E-GEOD-9078 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress