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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin immunoprecipitation of Streptomyces coelicolor HspR binding sites

ABSTRACT: Global ChIP-chip analysis of HspR repressor binding sites in Streptomyces coelicolor MT1110 cultures grown in YEME plus 10% sucrose at 30?C up to early stationary phase. Chromatin extracted from formaldehyde treated cultures was incubated with anti HspR polyclonal antibodies and the DNA purified from anti HspR IP and mock IP samples was labelled with either Cy3 or Cy5- dCTP and hybridized in an indirect and direct type of microarray experiment onto SCO-Chip2-v1 and SCO-Chip2-v2 High Density IJISS arrays, developed in collaboration with Oxford Gene Technology.

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Emma Laing

PROVIDER: E-MAXD-48 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Development and application of versatile high density microarrays for genome-wide analysis of Streptomyces coelicolor: characterization of the HspR regulon.

Bucca Giselda G Laing Emma E Mersinias Vassilis V Allenby Nicholas N Hurd Douglas D Holdstock Jolyon J Brenner Volker V Harrison Marcus M Smith Colin P CP

Genome biology 20090116 1

<h4>Background</h4>DNA microarrays are a key resource for global analysis of genome content, gene expression and the distribution of transcription factor binding sites. We describe the development and application of versatile high density ink-jet in situ-synthesized DNA arrays for the G+C rich bacterium Streptomyces coelicolor. High G+C content DNA probes often perform poorly on arrays, yielding either weak hybridization or non-specific signals. Thus, more than one million 60-mer oligonucleotide ...[more]

PMID: 19146703

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