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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Global distribution of RNA polymerase in Streptomyces coelicolor

ABSTRACT: Genome wide ChIP-chip analysis of RNA Polymerase binding sites in Streptomyces coelicolor MT1110 cultures grown in SMM + 0.5% casaminoacids at 30C up to mid exponential and stationary phase. Chromatin extracted from rifampicin and formaldehyde treated cultures was incubated with anti ? subunit of core RNA polymerase of E.coli monoclonal antibodies and the DNA purified from anti RNA Polymerase IP and mock IP samples was labelled with either Cy3 or Cy5- dCTP and hybridized in an direct type of microarray experiment onto SCO-Chip2-v2 High Density IJISS arrays

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Emma Laing

PROVIDER: E-MAXD-60 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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