Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of rat heart, kidney and placenta from fetuses obtained from dams with diet-induced intra-uterine growth restriction vs. normal

ABSTRACT: Pregnant rats were randomly divided into two groups. Dams were then housed individually and fed throughout gestation a 22 % protein diet, representing the control group (C, n=5), or an 9% isocaloric protein diet, representing the low-protein (LP, n=6). Both diets were isocaloric, because the protein deficiency in the LP diet was compensated by the addition of carbohydrates. Feeding of LP diet began on the day of conception and continuing throughout pregnancy. The low-protien diet resulted in Intra-Uterine Growth Restriction (IUGR) of the fetuses.

An additional processed data file, E-MEXP-1015_all_norm_calls.txt, is available for download from ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-1015/

ORGANISM(S): Rattus norvegicus

DISEASE(S): normal

SUBMITTER: Daniel Vaiman

PROVIDER: E-MEXP-1015 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A hierarchical analysis of transcriptome alterations in intrauterine growth restriction (IUGR) reveals common pathophysiological pathways in mammals.

Buffat C C Mondon F F Rigourd V V Boubred F F Bessières B B Fayol L L Feuerstein J-M JM Gamerre M M Jammes H H Rebourcet R R Miralles F F Courbières B B Basire A A Dignat-Georges F F Carbonne B B Simeoni U U Vaiman D D

The Journal of pathology 20071101 3

Intra-uterine growth restriction (IUGR) is a frequent disease, affecting up to 10% of human pregnancies and responsible for increased perinatal morbidity and mortality. Moreover, low birth weight is an important cause of the metabolic syndrome in the adult. Protein depletion during the gestation of rat females has been widely used as a model for human IUGR. By transcriptome analysis of control and protein-deprived rat placentas, we were able to identify 2543 transcripts modified more than 2.5 fo ...[more]

PMID: 17893880

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Project description:Gene expression studies in peripheral blood mononuclear cells (PBMC) can provide knowledge that would be difficult to obtain using other types of biological samples, as these cells can reflect overall response of the body to a specific stimulus, such as diet. Here, we aimed to study the impact of sustained intake of isocaloric diets with an unbalanced macronutrient proportion (rich in fat or protein) on PBMC gene expression in rats using transcriptomics, to better understand the effects of these diets on metabolism and health. Macronutrient diet composition (especially increased protein content) affected PBMC gene expression. An important effect was observed in immune response-related genes. High protein (HP) diet produced an activation of genes mainly related to antigen recognition/presentation, whereas the high fat (HF) diet was more related to a decreased expression of these genes. Key energy homeostasis genes (mainly involved in lipid metabolism) were also affected, reflecting a nutritional adaptive response to the diets. Decreased Dhrs3 and increased Pref-1/Dlk1 expression observed in HP-fed animals reflects a lower lipogenic capacity. In addition, HF diet affected the expression of genes related to insulin resistance/signaling (Msra/Slc9a6, Asb6), hypoxia (Akap12), cardiovascular problems (Ethe1, Lr11), cognitive impairment (Tmcc2) and tumorigenesis (Ddx17, Phb, Rnasen/Drosha, and Tsc2). HP diet also impaired the expression of genes related to glucose metabolism and insulinemia (Glut-4/Slc2a4, Sfxn5, Slc16a1), but positively affected genes involved in liver function (Clec4f and St6galnac3) and cardiovascular protection (Sort1). In conclusion, gene expression analysis in PBMC can provide information that could be useful to detect metabolic deviations and health consequences of diets with an unbalanced macronutrient composition. 2 months-old male Wistar rats received a low fat control diet, or a high fat (HF) diet, or a high protein (HP) diet for 4 months, respectively. At the end of the intervention, prior to the sacrifice of the animals, peripheral blood samples (1.5-2.5 mL) were collected from the safena vein, using heparin in NaCl (0.9%) as anticoagulant. Immediately after blood collection, peripheral blood mononuclear cells (PBMC) were isolated by Ficoll gradient separation. Total RNA was isolated from PBMC, quantified and qualified, and subsequently used for global gene expression profiling using Agilent 4x44K microarrays.

Dataset Information

Transcription profiling of rat heart, kidney and placenta from fetuses obtained from dams with diet-induced intra-uterine growth restriction vs. normal

Publications

A hierarchical analysis of transcriptome alterations in intrauterine growth restriction (IUGR) reveals common pathophysiological pathways in mammals.

Similar Datasets

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