Transcription profiling of pig alveolar macrophages infected with Porcine Respiratory Reproductive Syndrome Virus

ABSTRACT: A primary culture of Porcine Alveolar Macrophages (PAM) from six animals was split in two: one was infected at a multiplicity of infection (MOI) of 10 with a 13th passage of PRRSV Lelystad virus. The other culture was maintained as control. The percentage of infected cells ranged between 60 and 70% for all batches. Cells were collected 1, 3, 6, 9 and 12 hours Post Infection in Trizolfor RNA extraction. Total RNA extraction from PAM was performed with Trizol following standard instructions and clean-up was done using RNeasy columns. RNA quality was assessed by microcapillary electrophoresis on an Agilent 2001 Bioanalyzer with RNA 6000 Nanochips. RNA was quantified by spectrophotometry. Reverse transcription (RT) of 20 µg total RNA and synthesis of biotin-labeled cRNA with one round of a

ORGANISM(S): Sus scrofa

DISEASE(S): Control

SUBMITTER: Roberto Malinverni 

PROVIDER: E-MEXP-1350 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress