Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human breast carcinoma cells before and after snail induction to study the epithelial to mesenchymal cell phenotype conversion

ABSTRACT: We have applied our research on a well-documented biological model, the epithelial cell evasion mediated by the ectopic expression of the snail protein. Indeed, ectopic expression of Snail, a transcriptional repressor, is widely used to study the initial step of epithelial cell evasion also called Epithelial to Mesenchymal Transition (EMT). During this process, cells loose their intercellular junctions and acquired motility and an invasive behaviour. In order to have a temporal view of the gene expression modulation that occurred during EMT, we have established a biological model based on the conditional expression of snail protein in human non-invasive MCF-7 breast carcinoma cells that do not express endogenous snail and exhibit a well-organized epithelial phenotype. 96 hours after snail induction, the epithelial to mesenchymal cell phenotype conversion is completed.

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Homo sapiens

SUBMITTER: Arnaud Muller

PROVIDER: E-MEXP-1457 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of human breast carcinoma cells before and after snail induction to study the epithelial to mesenchymal cell phenotype conversion

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