Genotyping of S. aureus SH1000 transposon mutants to identify mutant positions

ABSTRACT: A library of transposon mutants were generated in S.aureus strain SH1000 using a custom Mariner transposon with an outward-facing T7 promoter. Genomic DNA was extracted from the library and digested with an appropriate restriction enzyme (AluI or RsaI). Labelled RNA run-offs were produced from the T7 promoter and hybridised to a tiling microarray to determine the position of the mutant. This was done using a library of a million S. aureus mutants, and genes not disrupted by a transposon were inferred to be essential for replication and growth in vitro.

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Roy Chaudhuri 

PROVIDER: E-MEXP-1588 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress