Transcription profiling of primary mouse hepatocytes reveals classification for genotoxic and non-genotoxic carcinogens by gene expression improves with exposure time

ABSTRACT: The prediction of possible carcinogenicity of chemicals for humans represents an ongoing challenge. Chronic rodent bioassays predict human cancer risk at only limited reliability while simultaneously being expensive and long-lasting. In order to seek for alternatives, in the present study, the ability of a transcriptomics-based primary mouse hepatocyte model to classify carcinogens by their modes of action was evaluated. As it is obvious that exposure will induce a cascade of gene expression modifications, in particular, the influence of exposure time in vitro on discriminating genotoxic (GTX) carcinogens from non-genotoxic (NGTX) carcinogens class prediction was investigated. Primary mouse hepatocytes from male C57Bl6 mice were treated for 12, 24, 36 and 48 h with two GTX and two NGTX ca

ORGANISM(S): Mus musculus

SUBMITTER: Danyel Jennen 

PROVIDER: E-MEXP-2209 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress